The aim of the experiment was to investigate the effect of dietary supplementation of yeast Saccharomyces cerevisiae on the growth performance of crossbred heifers. A total of eighteen (18) crossbred heifers, above 1 year of age (16-18 months) with similar body weights were selected for the experiment. The herd was divided into three groups, each consisting of 6 animals. The experimental groups were termed as control (T0), Treatment 1 (T1) and Treatment 2 (T2). The heifers of the experimental groups were individually fed a basal diet that consisted of a concentrate ration and roughages along with supplementation of 1.5gm/animal/day of dry yeast powder in T1 and 3gm/animal/day of dry yeast powder in T2 group respectively for a period of 90 days . The average initial and final body weight at 6 th fortnight of the heifers were found to be 188.18 ±1.07 , 188.32 ±0.89 . 188.61 ±0.79 kg and 216.41 ±1.18, 218.55 ±1.19 and 220.65 ±1.33 kg in T0, T1and T2 groups respectively. An increase of 28.23, 30.23 and 32.04 kg of body weight was in the respective groups. Analysis of variance of data did not imply any significant difference (P>0.05) of body weight and its interaction of the heifers in the experimental groups. The body weights of the heifers in all experimental groups increased linearly from 1st to 6th fortnight. The average mean daily body weight gain in T0, T1 and T2 were found to be 0.33 ± 0.04, 0.34 ±0.01, 0.35 ± 0.01kg in the 1st fortnight and 0.31 ± 0.05, 0.36 ±0.02 and 0.39 ±0.02 kg respectively in the 6th fortnight. The overall mean gain in daily body weight was observed to be 0.31 ± 0.03kg in T0 group, 0.35 ± 0.06kg in T1 and 0.36 ± 0.07kg in T2 group. The analysis of variance results showed that the mean average daily weight gain in T1 and T2 groups were significantly higher than T0. The results of one way analysis of variance showed that the average daily gain (ADG) to be significantly high (P<0.05) in T1 and T2 groups compared to T0 from 4 th to 6th fortnight. The overall mean fortnightly gain in body weight was significantly high (P<0.05) in T1 (4.94± 0.12kg) and T2 (5.31 ± 0.10 kg) groups as well as compared to T0 group(4.56 ± 0.04kg). With respect to individual fortnights, the average body weight gain was found to be higher in T1 and T2 groups than T0 group from 4th to 6th fortnight. The initial measurements of the body conformation traits in T0, T1 and T2 respectively were found to be 110.49±0.67, 110.89±0.42, 112.16±0.31 cm with respect to body length of heifers, 135.23±0.34, 135.45±0.21 and 135.52±0.16 cm with respect to heart girth and 107.51±0.21 ,107.95±0.25 and 108.51± 0.21cm with respect to height at wither and the final conformation traits of body length, hearth girth and height at wither were respectively recorded as 122.22±0.47 , 122.32±0.26 and 124.46±0.40 cm in T0, T1 and T2 respectively , 139.18±0.22 , 139.61±0.21 and 140.15±0.31 cm in T0, T1 and T2 respectively and 117.24±0.31 , 117.67±0.21 and 118.11±0.22 cm in T0, T1 and T2 respectively. The overall average values of the body conformation traits (cm) in T0, T1and T2 groups were found in accordance as 116.60±0.27 cm, 116.76±0.29 cm and 117.66±0.24 cm for body length, 136.96±0.12 , 137.29±0.99 , 137.69±0.13 cm for heart girth and 112.04±0.22 , 112.51±0.20 and 113.17±0.21 cm for height at wither . Increase in linear body measurements from 1st fortnight to 6th fortnight along with body weight was observed in all the three parameters. Analysis of data on body conformation traits showed no significant (P>0.05) difference in body length, hearth girth and height at wither. Also, all the parameters were seen to been higher in yeast supplemented group compared to control group. Further, correlation of the body measurements with body weight were studied and it was found that the linear body measurements were highly correlated (P<0.01) with body weight. The coefficient of correlation (r) of body length with body weight were found as 0.832, 0.907, 0.838 in T0, T1 and T2 respectively. With respect to heart girth, the coefficient of correlation with body weight was found to be 0.928, 0.931, 0.929 in T0, T1 and T2 respectively and the height at wither was found to be correlated to body weight as 0.915, 0.812 and 0.886 in T0, T1 and T2 respectively. The mean daily feed intake was found to be 4.37 ± 0.03, 4.39 ± 0.02 and 4.42 ± 0.02 kg/day in the 1st fortnight and 4.91 ± 0.03 , 4.93 ± 0.02 and 4.95 ± 0.03 kg/day in the 6th fortnight in T0, T1 and T2 respectively. The overall mean daily feed intake was found to be 4.66 ± 0.03, 4.68 ± 0.03 and 4.72± 0.02 kg/day in T0, T1 and T2 respectively. An increase in daily feed intake was observed successively in each fortnight. However, the results of the analysis of variance showed no significant (P>0.05) difference amongst the different experimental groups. With respect to the parameter of feed conversion efficiency, T1 and T2 showed better FCE ratio compared to T0. The FCE found in T0, T1 and T2 respectively in the 1st fortnight was 12.04 ±0.21, 12.22 ±0.30 and 11.58 ±0.35 and 12.03 ±0.32, 10.93 ±0.25 and 10.28 ±0.23 respectively in the 6th fortnight. The overall mean FCE observed in the three groups were 12.18 ±0.08, 11.45±0.22 and 10.93 ±0.19 in T0, T1 and T2 respectively. Results of analysis of variance revealed a highly significant (P<0.01) effect of supplementation of S.cerevisiae on the feed conversion efficiency of the experimental animals (T1 and T2 groups). A post-hoc test was further conducted and the results revealed that the FCE in T2 and T1 groups was significant from 3rd fortnight to 6th fortnight compared to the heifers of T0 group.