Bovine diarrhoea is considered as one of the most serious issue in the dairy industry, resulting in significant economic losses. A study was conducted in urban and peri-urban dairy farms of Meerut city of Uttar Pradesh state to isolate E. coli from diarrhoeic cattle, their molecular characterization and phylogenetic relationship. For the selection of the dairy farms or animals, the convenience sampling technique was used. A total of 47 faecal samples of diarrhoeic cattle were collected directly from the rectum. In this study, 32 (68.1%) samples were positive for E. coli using 16s ribosomal primer set, of which 02 were submitted for Sanger sequencing by outsourcing. The nucleotide sequences obtained from Sanger sequencing were subjected to BLAST in the NCBI database and result revealed one as Escherichia coli and one as Escherichia fergusonii. The sequences of both the isolates have been submitted to the NCBI database to obtain accession number. The phylogenetic tree constructed using partial 16S rDNA sequences of E. coli (MZ327277) and Escherichia fergusonii (MZ327276), showed that both the isolates obtained from this study shared mostly the same close relatives. The sequence similarity percentages of Escherichia fergusonii (MZ327276) when compared with other 16S rRNA gene sequences in Genbank were 99.33% to Escherichia fergusonii (MG457731), 99.33% to Escherichia coli, India. There is requirement of further investigation to determine the pathogenic potential of these serotypes of E. coli with more number of samples.
Dolomite enriched with nitrogen, phosphorus and potassium (trade name Fertdolomite: 24.0% CaO + 16.0% MgO + 3.0% N + 2.5% P2O5 + 3.0% K2O) was applied in autumn of 2011 in three rates (3.56, 7.14 and 14.25 t ha-1) with aim of testing impact on maize and winter wheat status in crop rotation on Gorjani acid soil (pH in 1nKCl = 4.13; hydrolitical acidity 6.78 cmol kg-1) of Osijek-Baranya County. \nIn 2012 the maize yield was pretty low (2.71 t ha-1), which was mainly caused by high contribution of barren plants due to drought high air-temperature stress in maize flowering. The average maize grain yield under normal weather conditions of 2014 was 12.15 t/ha and by Fertdolomite yield was significantly increased by 11%. \nIn the 2012/2013 growing season as affected by Fertdolomite wheat yield was increased by 22% (6.93 and 8.47 t ha-1, respectively) and in 2015 by 25% (6.46 and 8.08 t ha-1, respectively). By the highest Fertdolomite rate were significantly increased grain quality parameters of wheat (2-year averages: comparison with the control), for example protein (10.8% and 11.9%, respectively), wet gluten (27.1% and 30.1%, respectively) and sedimentation (32.3 ml and 38.2 ml), while differences in other parameters (thousand grain weight, hectoliter mass and starch contents) were within statistical error (Table 4). However, these quality parameters were under considerable influences of growing season specifies (2013 and 2015, respectively) as follows: 10.8% and 11.9% (protein contents), 26.9% and 30.9% (wet gluten), 31.0 ml and 39.6 ml (sedimentation).
Adverse effects are important factors that threaten the survival and quality of life of humans; it can impose great cost to the health system of a country. Co-trimoxazole is an antibiotic which is achieved by the combination of sulfamethoxazole and trimethoprim. The drug, like other types of antibiotics has many side effects on different body systems. This report is about 30 year old woman, who referred to the emergency department followed by numbness of the tongue after taking co-trimoxazole. The patient didn\'t take other medications during the past month and this drug has been prescribed for colds and clinical symptoms have shown after taking 8 of the drug. The patient had other complications that after receiving oxygen and outpatient treatment, all symptoms resolved, except for numbness of the tongue. After 10 days, the problem was solved. It is necessary that respected doctors should also consider the complication related to the numbness of the tongue when prescribing co-trimoxazole, which is also widely used drug.
In this paper we developed a module helps NC programming instructions machining standard in CAM environment. This module allows learning to numerically as well as conversational programming mode. Two parties have been developed. The first deals with the problem of incompatibility of functions preparatory commands FANUC, FAGOR, NUM, SINUMERIK in the Turning and milling. In the second part, it was developed a module contributing to the adaptation of standard machining instructions in CAM environment.
A 23-year-old female presented with left gluteal pain and limping for one year after motorcycle accident. Examination showed tip-toeing antalgic gait, left lower limb was internally rotated with 2.8 cm shortening without neurovascular impairment. Radiograph revealed posterior dislocation with fracture of the roof and posterior column of the acetabulum. Hence gradual traction was commenced up to 14.6 kg within a month. Open reduction was done but abduction cast was applied to keep the left hip externally rotated and abducted. \nOn follow up she was able to ambulate after 2 months. MRI after 4 months suggested sign of osteonecrosis of the superior head and 8 months later she been complaining of anterior hip and knee pain on full weight bear without instability. She had started working but requires occasional analgesia. The chronological and related future problems with further treatment options had been well informed to patient.
This paper presents an investigation of the surface roughness in a turning process of SAE 1020 and SAE 1045 materials in order to suggest experimental models. The main objectives are to predict the surface roughness, to select the optimal cutting parameters, and to analyze the effects of cutting parameters. The study of this work allows to find more efficient methods to assist the Computer Numeric Control (CNC) turning process, optimizing its cutting parameters: cutting speed, feed rate and depth of cut. Factorial design was used for the experiment plan. At work, we investigated the turning conditions for SAE 1020 and 1045 steels with carbide tool. The measured data were analyzed by regression analysis. From the experimental results and regression analysis, this research project suggested the experimental equations, proposed the optimal cutting parameters, and analyzed the effects of cutting parameters on surface roughness.
Fungicides act as stress factors, altering the microbiological balance in soils. The present study quantified the effect of difenoconazole (DFC) on bacteria and mold communities grown in laboratory conditions, in treated soil microcosms, using the following concentrations: control, half dose (0.037 mg DFC g-1 soil, HD), normal dose (0.075 mg DFC g-1 soil, ND) and double dose (0.150 mg DFC g-1 soil, DD). The microbiological analyses included mesophilic bacteria involved in the nitrogen cycle: aerobic and anaerobic nitrogen-fixing, ammonifying, nitrifying and denitrifying bacteria. The mesophilic bacteria were incubated at 37°C and identified qualitatively on solid growth medium (nutritive gelose), whereas soil fungi were identified both quantitatively and qualitatively on solid growth medium (Potato-Glucose-Agar). The noticeable effects of DFC were mainly focused on soil microbial communities, negatively affecting both the quantity and the quality of soil microorganisms. In high doses, the DFC decreased the microbial communities involved in nitrogen cycle, namely aerobic and anaerobic bacteria and denitrifying bacteria. Noticeable was the response of two genera, namely Mucor sp. and Actinomucor sp., that showed resistance to the toxic effect of DFC, underlying their potential further practical use in the decontamination of polluted soils.
Slovenia as one of the European Union member states has a very high percentage of children included in organized forms of preschool education (percentage amounting to 88,5% for the year 2015/16). In the country, inclusive and special, supportive and rehabilitation programs for diverse groups of preschool and school children with special needs (CSN) are carried out. The Slovenian educational legislation enables CSN to enter one of the two preschool programs. The first one is adapted preschool program with additional professional assistance, taking place in regular kindergarten classes. The second one is adapted preschool program; it takes place in the do-called developmental kindergarten classes and is intended for children with pronounced developmental deficiencies. Both programs enable maximum special-pedagogical and social adaptation to the child’s needs and abilities.\nThe study has proven that the legislation change and reorganization of placement procedures have no major impact. A trend of constant increase of inclusion of CSN (throughout the monitored five year period) into the regular kindergarten classes can be noticed. The study confirmed a slight connection between the number of placed CSN and the number of residents in an individual regional unit as well as between the placed CSN and the number of live-born children in 2015/16. Our study proves that continuous, intensive inclusion processes, enabling an earlier and better detection of CSN, are present in the country for a lengthy period of time. Also CSN with more prominent developmental deficiencies are mainly included in regular kindergarten classes, where they get an optimal support.