Background\nThe main idea is directed to development of maximally safe and assessable methods for derivation of various normal mature cell types from stem/progenitor cells by application of appropriate laboratory incubation techniques. The last could vary, depending both of the respective tools of stem/progenitor cells, as well as of the respective wished mature cell type.\nMethods\n3T3 normal mouse embryonic cells were incubated in cultural fluid, supplemented by previous cultivation of malignant mouse myeloma cells in it (after centrifugation and filtration). Separated sub-populations of these cells were treated with cryo-protector Dimethylsulfoxide (DMSO), freezed, thawed and re-cultivated. Another 3T3 sub-population was de novo-pre-cultivated in cultural fluid, supplemented after previous incubation of osteoclast-like cells or of myeloid progenitors in it. 3D-visualization technology, combined with laser irradiation, were developed and proved as usable for these pilot studies, which are initial steps of investigations, allowing the determination of presence and/or localization of respective molecules in the complex structures, by intra- and extra-cellular inter-molecular interactions.\nResults and Discussion\nAfter addition of respective volume DMSO, freezing and subsequent thawing and re-cultivation, derivation of multi-nuclear osteoclast-like cells was noted. The results obtained were confirmed by the observed appearance of osteoblast-like cells in incubation of de novo-formed monolayer of 3T3 fibroblasts in cultural fluid from the so derived osteoclast-like cells, described above, but also by the observed destruction of the last in co-cultivation of cells from both sub-types derived (cultural fluids plus cell suspensions). These changes could explain with the existence of stem-like cells in the general 3T3 cell line, and this explanation could be confirmed by the observed signs of early myeloid differentiation of these cells in their short-term incubation in cultural fluid from mouse malignant myeloid cells in the absence of DMSO, as well as freezing and thawing procedures. According to the results obtained, besides the factors of cultivation and the components of extra-cellular matrix, the role of cell receptors and organic detergents on the cell differentiation direction, but also of intra-cellular fusion in the formation of osteoclast-like cells, was shown. Hence, in agreement with the literature data, a probability about the influence of DMSO and other organic detergents on gene transfer was proposed.\nConclusions\nDifferent lineages of normal mature cells could be derived from progenitors with common source by appropriate incubation. Also, the combined technique of laser irradiation of biological objects with their 3D-visualization gives a possibility about more precise identification of the changes, occurring in different phases of living processes, on both cellular and organism levels.